Determination of the lethal concentration 50% (LC50) of lead chloride and its accumulation in different organs of Gambusia affinis fish

In the present research, mosquito fish Gambusia affinis have been exposed to lead chloride during 24, 48, 72 and 96 hours in order evaluate lethal 50 (LC50) concentration Its residue certain organs of fish. Usage EPA computer software based on Finney Probit Analysis method has statistically tested for data collected LC50 values G. if were found be 59.4, 55.9, 51.1 49. 0 mg/L, respectively. decreased as mean exposure times. 20 placed each four sublethal concentrations 25 mg/L two acute periods 24 well 10 5 mg/l chronic 15 30 hours. The testes carried out three replications, accumulation various was determined by Atomic Absorption Spectrophotometer. finding revealed that PbCl2 different time dependent fashion Pb-content increased significantly at compared acute- exposure.